Caspase 3 (CASP3) Mouse Monoclonal Antibody [Clone ID: 31A1067]

Specifications

Product Data
Clone Name	31A1067
Applications	CyTOF-ready, Electron Microscopy, FC, ICC/IF, IHC, Immunoblotting, Simple Western, WB
Recommend Dilution	Immunohistochemistry: 1:10 - 1:500, Simple Western: 1:50, Western Blot: 1 - 5 ug/ml, Knockout Validated, Knockdown Validated, Immunohistochemistry-Frozen: 1:10 - 1:500, Immunohistochemistry-Paraffin: 1:10 - 1:500, Immunocytochemistry/ Immunofluorescence, Flow Cytometry, Electron Microscopy, Immunoblotting, Hematoxylin and Eosin Stain, Immunohistochemistry Free-Floating, CyTOF-ready
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	caspase 3
Database Link	NP_116786 Entrez Gene 12367 MouseEntrez Gene 25402 RatEntrez Gene 836 Human UniProt ID P42574
Background	Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.
Synonyms	CPP32; CPP32B; SCA-1
Note	Useful in Immunohistochemistry-Frozen See Zhang et al., and Immunohistochemistry-Paraffin See Lee et al. Use in Immunocytochemistry/Immunofluorescence was reported in the scientific literature (PMID: 23840553).
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Protease
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis

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