GAPDH Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Immunoblotting, Simple Western, WB
Recommend Dilution	Simple Western: 1:500, Immunohistochemistry, Western Blot: 1:500-1:2000, Immunoblotting, Immunocytochemistry/ Immunofluorescence
Reactivity	Human, Mouse, Rat, Drosophila, Feline, Porcine, Protozoa
Host	Rabbit
Clonality	Polyclonal
Immunogen	Amino acids 73-87 PITIFQERDPSKIKW of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	glyceraldehyde-3-phosphate dehydrogenase
Database Link	NP_002037 Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 2597 Human UniProt ID P04406
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Synonyms	G3PD; GAPD; HEL-S-162eP
Reference Data
Protein Families	ES Cell Differentiation/IPS
Protein Pathways	Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

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