p16INK4A (CDKN2A) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry: 10 ug/ml, Immunohistochemistry-Paraffin: 10 ug/ml, ELISA: 1:100-1:2000, Immunocytochemistry/ Immunofluorescence: 1:50 - 1:100, Western Blot: 2 ug/ml, Immunohistochemistry-Frozen: 1 ug/ml
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p19ARF. [Swiss-Prot #P51480]
Formulation	PBS, 0.02% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	19 kDa
Gene Name	cyclin-dependent kinase inhibitor 2A
Database Link	NP_000068 Entrez Gene 12578 Mouse UniProt ID Q8N726
Background	The ARF gene, p19ARF in mouse and p14ARF in human, has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53 dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction.
Synonyms	ARF; CDK4I; CDKN2; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4
Note	This p19ARF antibody is useful for ELISA, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Western Blot. In WB a band can be seen at approximately 19 kDa.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer

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