NuMA (NUMA1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence: 1:500, Western Blot: 1:1000, Simple Western: 1:500, Immunohistochemistry: 5 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml, Immunoprecipitation: 1:10-1:500
Reactivity	Human, Mouse, Rat, Mammalian, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system, then purified to near homogeneity prior to immunization.
Formulation	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	nuclear mitotic apparatus protein 1
Database Link	NP_001273490 Entrez Gene 101706 MouseEntrez Gene 308870 RatEntrez Gene 4926 Human UniProt ID Q14980
Background	NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning. Therefore, this antibody is useful as a cell cycle marker.
Synonyms	NMP-22; NUMA
Note	This NuMA antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot, where a band can be seen at 220 kDa. Immunohistochemistry-Frozen was reported in scientific literature.
Reference Data

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