beta Actin (ACTB) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Block/Neutralize, ELISA, FC, ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Immunohistochemistry-Paraffin: 1:100, Proximity Ligation Assay, Simple Western: 1:12.5, Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation: 1:10-1:500, Western Blot: 1:100-1:2000, Flow Cytometry: 1:10-1:1000, Block/Neutralize, ELISA
Reactivity	Human, Mouse, Rat, Avian, Chinese Hamster, Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an N-terminal region of human Beta Actin. [UniProt P60709].
Formulation	Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	actin, beta
Database Link	NP_001092 Entrez Gene 11461 MouseEntrez Gene 81822 RatEntrez Gene 60 Human UniProt ID P60709
Background	Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta actin and the static and dynamic aspects of the cytoskeleton.
Synonyms	BRWS1; PS1TP5BP1
Note	Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Immunocytochemistry/Immunofluorescence, Blocking/Neutralizing and Enzyme Immunoassay were reported in scientific literature. Customer review using ICC. Use in Proximity Ligation Assay reported in scientific literature (PMID 24740405)
Reference Data
Protein Families	ES Cell Differentiation/IPS
Protein Pathways	Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis

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