TNF alpha (TNF) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:200 - 1:300, Flow Cytometry, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 1:200, Western Blot: 1:250 - 1:500, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of the human TNF alpha protein (between residues 100-200) [UniProt P01375]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	tumor necrosis factor
Database Link	NP_000585 Entrez Gene 21926 MouseEntrez Gene 24835 RatEntrez Gene 7124 Human UniProt ID P01375
Background	Tumor Necrosis Factor Alpha (TNF alpha) is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages and causes tumor necrosis when injected into tumour bearing mice. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid etabolism, and coagulation and it has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the europrotective function of this cytokine. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Synonyms	DIF; TNF-alpha; TNFA; TNFSF2
Note	Western Blot data from customer review and citation (PMID: 24223475). Predicted reactivity: Porcine (100%), Canine (90%), Feline (100%), Mustelid (90%), Primate (90%).
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways	Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus

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