Calreticulin (CALR) Mouse Monoclonal Antibody [Clone ID: 1G6A7]

Specifications

Product Data
Clone Name	1G6A7
Applications	ELISA, FC, ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry: 1:200 - 1:1000, Immunohistochemistry-Paraffin: 1:200 - 1:1000, ELISA: 1:1000, Flow Cytometry: 1 ug/ml, Flow (Intracellular): 1 ug/ml, In vitro assay, Western Blot: 1:500 - 1:2000, Immunocytochemistry/ Immunofluorescence: 1:200 - 1:1000
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide corresponding to the C-terminus (EEEDVPGQAKDELC) of human Calreticulin, conjugated to KLH. [UniProt# P27797]
Formulation	PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ammonium sulfate precipitation
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	48 kDa
Gene Name	calreticulin
Database Link	NP_004334 Entrez Gene 12317 MouseEntrez Gene 811 Human UniProt ID P27797
Background	Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however, it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone.
Synonyms	cC1qR; CRT; HEL-S-99n; RO; SSA
Note	This Calreticulin (1G6A7) antibody is useful in Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transcription Factors
Protein Pathways	Antigen processing and presentation

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