CX3CL1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry: 1:250, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry-Paraffin: 1:250, Western Blot, Immunohistochemistry-Frozen
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A genomic peptide made to an internal region of the human CX3CL protein (within residues 20-150). [Swiss-Prot P78423]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	C-X3-C motif chemokine ligand 1
Database Link	NP_002987 Entrez Gene 20312 MouseEntrez Gene 6376 Human UniProt ID P78423
Background	As the only member of intercrine delta/ CX3C family, CX3CR1 (C-X3-C motif chemokine 1) is a type I transmembrane protein with CX3C chemokine domain present on an extended glycosylated stalk and it share this unique membrane anchorage with CXCR6 ligand, CXCL16. CX3CL1 is constitutively shed from the cell surface by ADAM10 (A Disintegrin and Metalloprotease 10) and CX3CL1 cleavage is enhanced upon activation of the closely related protease TNFalpha-converting enzyme, TACE (ADAM-17). CX3CL1 is expressed in a wide variety of cell types including neurons, intestinal , airway , skin epithelium cells, airway smooth muscle cells, and endothelium. It is induced by TNF and IL1 in pulmonary endothelial cells and umbilical vein endothelial cells. CX3CL1 signals through CX3CR1 (the only receptor of CX3CL1) which is expressed on monocytes, natural killer cells, T cells and smooth muscle cells. The major function of CX3CL1's soluble form is to act as chemotactic for T-cells and monocytes, but not for neutrophils, whereas, the membrane-bound form promotes adhesion of those leukocytes to endothelial cells where it may play a role in regulating leukocyte adhesion and migration processes at the endothelium. In addition to its role in chemotaxis and adhesion of leukocytes, CX3CL1 implicates in survival of multiple cell types during homeostasis and inflammation.
Synonyms	ABCD-3; C3Xkine; CXC3; CXC3C; fractalkine; neurotactin; NTN; NTT; SCYD1
Note	This CX3CL1 antibody is useful for IHC and ICC. Immunohistochemistry-Frozen was reported in scientific literature.
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.