Aurora A (AURKA) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Western Blot: 1 - 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 2 ug/ml, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 1:200, Simple Western: 1:200
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A genomic peptide made to an N-terminal region of the human Aurora A protein (within residues 50-200). [Swiss-Prot O14965]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	aurora kinase A
Database Link	NP_940835 Entrez Gene 20878 MouseEntrez Gene 6790 Human UniProt ID O14965
Background	Aurora A is a cytoplasmic serine/threonine kinase that is critical in transitioning cells through mitosis and meiosis. Aurka acts through phosphorylating several spindle and tubulin proteins. Without Aurka, axon and neuron formation are negatively impacted. When these critical cellular processes are disrupted, there is a potential for tumorgenesis, and thus Aurka is implicated in many cancers, particularly those of the blood. In studies of angiofibromas, both MDM1 and Aurka were amplified. Overall, cell proliferation seems proportional to the expression of Aurka.
Synonyms	AIK; ARK1; AURA; BTAK; PPP1R47; STK6; STK7; STK15
Note	This AURKA antibody is useful for Immunohistochemistry and Western blot, where a band is seen ~45 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways	Oocyte meiosis

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