iNOS (NOS2) Mouse Monoclonal Antibody [Clone ID: 4E5]

Specifications

Product Data
Clone Name	4E5
Applications	ELISA, FC, IHC, WB
Recommend Dilution	Immunohistochemistry-Frozen, Immunohistochemistry, Flow (Intracellular), Flow Cytometry: 1:200-1:400, Immunohistochemistry-Paraffin: 1:200-1:1000, ELISA: 1:10000, Western Blot: 1:500-1:2000
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human iNOS expressed in E. coli. [UniProt# P35228]
Formulation	PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ammonium sulfate precipitation
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	131 kDa
Gene Name	nitric oxide synthase 2
Database Link	NP_000616 Entrez Gene 18126 MouseEntrez Gene 4843 Human UniProt ID P35228
Background	Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Synonyms	HEP-NOS; INOS; NOS; NOS2A
Note	This iNOS (4E5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer

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