Myelin Basic Protein (MBP) Mouse Monoclonal Antibody [Clone ID: 2H9]

Specifications

Product Data
Clone Name	2H9
Applications	ELISA, FC, ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry-Frozen, Immunohistochemistry, Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Western Blot: 1:500-1:2000, Immunohistochemistry-Paraffin: 1:200-1:1000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human Myelin Basic Protein expressed in E. coli. [UniProt# P02686]
Formulation	PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ammonium sulfate precipitation
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	33 kDa
Gene Name	myelin basic protein
Database Link	NP_001020252 Entrez Gene 4155 Human UniProt ID P02686
Background	The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
Synonyms	MGC99675
Note	This Myelin Basic Protein (2H9) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.
Reference Data

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