Sonic Hedgehog (SHH) Mouse Monoclonal Antibody [Clone ID: 5H4]

Specifications

Product Data
Clone Name	5H4
Applications	ELISA, FC, ICC/IF, IHC, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence, Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Western Blot: 1:500-1:2000, Immunohistochemistry-Paraffin: 1:200-1:1000, Immunohistochemistry
Reactivity	Human, Mouse, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human Sonic Hedgehog (amino acids 26-170) expressed in E. coli. [UniProt# Q15465]
Formulation	Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ascites
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	49.6 kDa
Gene Name	sonic hedgehog
Database Link	NP_000184 Entrez Gene 20423 MouseEntrez Gene 6469 Human UniProt ID Q15465
Background	This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.
Synonyms	HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS
Note	This Sonic Hedgehog (5H4) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA.
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways	Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer

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