H4-16 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Dot, ELISA, IF, WB
Recommend Dilution	ChIP/ChIP-seq (0.5-1ug/IP); ELISA (1:1000); Dot blotting (1:20,000); Western blotting (1:1,000); Immunofluorescence (1:500)
Reactivity	Human, Mouse, Broad
Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen for anti-H4K5,8,12ac antibody: the region of histone H4 containing the acetylated lysines 5, 8 and 12 (H4K5,8,12ac), using a KLH-conjugated synthetic peptide.
Concentration	lot specific
Purification	Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	histone cluster 4, H4
Database Link	NP_778224 Entrez Gene 320332 MouseEntrez Gene 121504 Human UniProt ID P62805
Background	Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H4 is associated with active genes.
Synonyms	H4; p
Reference Data
Protein Pathways	Systemic lupus erythematosus

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