ARHI (DIRAS3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1 - 2 ug/mL
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	DIRAS3 antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 25 kDa; Observed: 24 kDa
Gene Name	DIRAS family GTPase 3
Database Link	NP_004666 Entrez Gene 9077 Human UniProt ID O95661
Background	DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).
Synonyms	CACNA1HB; Cav3.2; ECA6; EIG6; HALD4
Reference Data

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