NF-kB p65 (RELA) Rabbit Polyclonal Antibody

Specifications

Product Data
Recommend Dilution	WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
Reactivity	Human, Rat, Rabbit
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide derived from internal domain of human NF?B p65.
Formulation	This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Purification	The Rabbit IgG is purified by Epitope Affinity Purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	65 kDa
Gene Name	RELA proto-oncogene, NF-kB subunit
Database Link	NP_001138610 Entrez Gene 309165 RatEntrez Gene 5970 Human UniProt ID Q04206
Background	NF-?B (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls the transcription of DNA. NF-?B is important in regulating cellular responses because it belongs to the category of "rapid-acting" primary transcription factors. NF kappa B is a heterodimer consisting of a 50kDa DNA binding subunit and a 65kDa transactivating subunit. In unstimulated cells, the NF-?B dimers are sequestered in the cytoplasm by a family of inhibitors, called I?Bs (Inhibitor of ?B). Activation of the NF-?B is initiated by the signal-induced degradation of I?B proteins. Subsequent to cell stimulation, I?B undergoes phosphorylation, ubiquitination and degradation by a proteosome-dependent pathway, allowing nuclear translocation of the active dimeric NF?B transcription factor. NF-?B complex enters the nucleus where it can 'turn on' the expression of specific genes that have DNA-binding sites for NF-?B nearby. The activation of these genes by NF-?B then leads to the given physiological response, for example, an inflammatory or immune response, a cell survival response, or cellular proliferation.
Synonyms	NFKB3; p65
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway

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