GAPDH Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 0.5-1µ/ml.
Reactivity	Human, Mouse, Rat, Rabbit, Chicken
Host	Rabbit
Clonality	Polyclonal
Immunogen	Biotin-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
Formulation	Biotin-GAPDH antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	Biotin-GAPDH antibody is affinity chromatography purified via peptide column.
Conjugation	Biotin
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 37 kDa; Observed: 36 kDa
Gene Name	glyceraldehyde-3-phosphate dehydrogenase
Database Link	NP_002037 Entrez Gene 2597 Human UniProt ID P04406
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Synonyms	G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432
Note	Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 µ/ml.
Reference Data

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