alpha Tubulin (TUBA1A) Mouse Monoclonal Antibody [Clone ID: 2B11]

Specifications

Product Data
Clone Name	2B11
Applications	WB
Recommend Dilution	WB: 1-2µg/ml.
Reactivity	Human, Mouse, Rat, Rabbit, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin
Formulation	alpha-Tubulin antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	alpha-Tubulin antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 50 kDa; Observed: 54 kDa
Gene Name	tubulin alpha 1a
Database Link	NP_006000 Entrez Gene 22142 MouseEntrez Gene 64158 RatEntrez Gene 7846 Human UniProt ID Q71U36
Background	Alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
Synonyms	B-ALPHA-1; FLJ25113; hum-a-tub1; hum-a-tub2; LIS3; TUBA3
Note	alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 µg/ml.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Gap junction, Pathogenic Escherichia coli infection

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