A2M Mouse Monoclonal Antibody [Clone ID: PAP-F101]

Specifications

Product Data
Clone Name	PAP-F101
Applications	IHC
Recommend Dilution	IHC (f), WB (native). Each lot has been tested and validated for immunohistochemistry (IHC). Approximate working dilution for IHC: Frozen sections: 0.2-0.4µg/ml (1:1000-1:2000) Paraffin sections: does not react on routinely processed paraffin sections. Optimal dilutions should be determined by the end user. Suggested positive control: swine kidney.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine lung extract.
Specificity	Porcine: positive. Other: not tested Epitope: The antigen is alpha-2-Macroglobulin, according to the results obtained in IHC and in Western Blot with purified α-2-macro- globulin. The epitope has not been further characterized. Distribution: Tissue sections: The antibody reacts with tissue sections of kidney, pancreas, brain, stomach, adrenal, lung, intestine, skin, testis, liver, lymph node and uterus. Staining is prominent with endothelial structures in all organs, and with serum.
Formulation	Affinity purified from cell culture supernatant, lyophilized. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Minimize repeated thawing and freezing of the stock solution.
Background	Clone PAM-F101 is a unique monoclonal antibody developed against porcine α-2-Macro- globulin. α-2-Macroglobulin (α2M) is a broad spectrum proteinase inhibitor synthesized mainly by hepatocytes, and locally by macrophages. The inhibitory mode of action uses a capturing mechanism through a peptide sequence (“bait region”) which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change and concomitant thioester bond hydrolysis is induced, leading to covalent binding of α2M to the proteinase. The bound enzyme remains active against low molecular weight substrates while the activity against high molecular weight substrates is reduced. The proteinase-α2M complex is recognized by macrophage receptors and cleared from the system. α2M is composed of four identical subunits arranged as a pair of disulfide-linked dimers, altogether with 720kDa molecular weight. α2M is sensitive to hypochlorite which induces dissociation of native α2M tetramers into stable dimers that are no longer able to trap proteases. Electrophoresis typically yields a 360kDa band in the native state, 180kDa with SDS-PAGE under non-reducing conditions. Reducing conditions (e.g. dithiothreitol DTT) will generate two different fragments with molecular mass of 93 and 87 kDa, respectively. This antibody was produced serum-free, without fetal calf serum.
Reference Data

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