Bronchial Basement Cells Mouse Monoclonal Antibody [Clone ID: PLB42]

Specifications

Product Data
Clone Name	PLB42
Applications	ELISA, IHC, WB
Recommend Dilution	Tested for immunohistochemistry (IHC), other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 1µg/ml (1:500) IHC, paraffin sections: 2.5µg/ml (1:200) Proteinase K pretreatment for antigen retrieval is recommended. Optimal dilutions should be determined by the end user.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine lung extract.
Specificity	Pig: bronchial basement cells, Leydig cells Other species: reacts with human tissue Epitope: The antigen has not yet been identified. The immunohistochemical staining pattern doesn’t match other well- known basal cell markers. Further characterization is under way. Distribution: Tissue sections: The antibody reacts with bronchial basement cells of the lung and Leydig cells in the testes.
Formulation	Affinity purified from cell culture supernatant, lyophilized. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.5mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Avoid repeated thawing and freezing of the reconstituted antibody.
Background	Monoclonal antibody PLB42 is a particularly interesting marker of bronchial basement cells of the lung epithelium. The prominent staining of basal cells clearly divides the pseudostratified columnar epithelial cell layer from the underlying basement membrane and lamina propria. A subpopulation of basal cells probably carries stem cell characteristics and is involved in cell replacement. Other comparable structures such as the basement cells of the intestinal epithelium are not specifically stained. However, Leydig cells (also known as interstitial cells of Leydig) in the testes are stained positively. This antibody was produced serum-free, without fetal calf serum.
Reference Data

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