Reticular Fibroblasts and Reticular Fibres Mouse Monoclonal Antibody [Clone ID: PRF414]

Specifications

Product Data
Clone Name	PRF414
Applications	ELISA, IHC
Recommend Dilution	Tested for immunohistochemistry (IHC), other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.5µg/ml (1:800) IHC, paraffin sections: 2µg/ml (1:200) Proteinase K pretreatment for antigen retrieval is recommended. Optimal dilutions should be determined by the end user.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine lung extract.
Specificity	Pig: reticular fibres Other species: Does not react with human tissue. Epitope: The epitope has not been characterized. Distribution: Tissue sections: In all organs tested so far, the antigen is expressed in connective tissues which form a supporting network between parenchymal cells. Thus, the supportive mesenchymal structures of vessels and the capillary structure of tissues can be studied. In the spleen, a very clear delineation of red and white pulp is obtained. Capsule, sinuses, follicles, paracortex and medullary cords are also clearly delineated in lymph nodes.
Formulation	Affinity purified from cell culture supernatant, lyophilized. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Avoid repeated thawing and freezing of the reconstituted antibody.
Background	Monoclonal antibody PRF414 detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The reticular fibres are composed of collagen fibrils, predominantly type III collagen. This antibody was produced serum-free, without fetal calf serum.
Reference Data

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