ATP4A Monoclonal Antibody [Clone ID: PPC19]

CAT#: TA363882

H,K-ATPase alpha, (ATP4A), mouse anti porcine, clone PPC19



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CNY 9,994.00


货期*
5周

规格
    • 200 ug

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Specifications

Product Data
Clone Name PPC19
Applications ELISA, IHC, WB
Recommend Dilution Each lot of this antibody has been tested and validated for immunohistochemistry on formalin-fixed paraffin sections (IHC-P). Approximate working dilution for IHC: Paraffin sections: 4-8 µg/ml (1:50-1:100); microwave pretreatment for antigen retrieval is recommended.
Reactivity Pig
Clonality Monoclonal
Immunogen Porcine stomach extract.
Specificity Pig: parietal (oxyntic) cells Other: not tested

Epitope: The antibody reacts with formaldehyde-treated porcine gastric H,K-ATPase. The epitope has not been further characterized.

Distribution: Tissue sections: porcine gastric parietal (oxyntic) cells of the fundic gland region
Formulation State: Affinity purified from cell culture supernatant, lyophilized, packaged under nitrogen.
Buffer: Phosphate buffered saline pH 7.2 (PBS), 6 mg/ml BSA as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
Reconstitution Method Reconstitute by adding 0.5 ml distilled water.
Concentration 0.4 mg/ml IgG after reconstitution
Conjugation Unconjugated
Storage Condition Original vial: 1 year at 4° - 8°C. Minimize repeated thawing and freezing of the stock solution.
Background Monoclonal antibody PPC19 recognizes the porcine gastric H,K-ATPase. This enzyme catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane and is thus responsible for acid production in the stomach. The functional proton pump is actually a heterodimer consisting of a 114kDa alpha subunit with catalytic activity, and a 33kDa stabilizing beta subunit. Of the various cell types that form the gastric mucosa, only parietal (oxyntic) cells of the fundic gland region possess this enzyme. Of pathological significance, their dysfunction induced by H. pylori infection leads to atrophic gastritis. This antibody was produced in vitro in bioreactors.
Synonyms ATP6A
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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