EIF3B Rabbit Monoclonal Antibody [Clone ID: R09-2A9]

Specifications

Product Data
Clone Name	R09-2A9
Applications	IHC, WB
Recommend Dilution	WB: 1/1000-1/5000 IHC: 1/20-1/50
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human eIF3B
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 93 kDa; Observed MW: 117 kDa
Gene Name	eukaryotic translation initiation factor 3 subunit B
Database Link	Entrez Gene 8662 Human UniProt ID P55884
Background	Swiss-Prot Acc.P55884.RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Synonyms	eIF-3-eta; EIF3-ETA; EIF3-P110; EIF3-P116; EIF3S9; hPrt1; MGC104664; MGC131875; PRT1
Reference Data

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