GRP78 (HSPA5) Rabbit Monoclonal Antibody [Clone ID: R03-5B3]

Specifications

Product Data
Clone Name	R03-5B3
Applications	IHC, WB
Recommend Dilution	WB: 1/1000-1/5000 IHC: 1/50-1/200
Reactivity	Human, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human GRP78 BiP
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 72 kDa; Observed MW: 78 kDa
Gene Name	heat shock protein family A (Hsp70) member 5
Database Link	Entrez Gene 3309 Human UniProt ID P11021
Background	Swiss-Prot Acc.P11021.Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:2294010, PubMed:23769672, PubMed:23990668, PubMed:28332555). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate . Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:1550958, PubMed:19538957). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 . Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 . Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating.
Synonyms	BiP; FLJ26106; GRP78; MIF2
Reference Data

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