PAH Rabbit Monoclonal Antibody [Clone ID: R02-8B1]

Specifications

Product Data
Clone Name	R02-8B1
Applications	IF, IHC, IP, WB
Recommend Dilution	WB: 1/1000-1/5000 IHC: 1/20-1/100 ICC/IF: 1/20-1/50 IP: 1/20
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human PABPN1
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 33 kDa; Observed MW: 50 kDa
Database Link	UniProt ID Q86U42
Background	Swiss-Prot Acc.Q86U42.Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product . Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length . Increases the affinity of poly(A) polymerase for RNA . Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity . May protect the poly(A) tail from degradation . Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484).
Reference Data

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