STAT1 Rabbit Monoclonal Antibody [Clone ID: R07-7A3]

Specifications

Product Data
Clone Name	R07-7A3
Applications	IF, IP, WB
Recommend Dilution	WB: 1/2000-1/10000 ICC/IF: 1/500-1/1000 IP: 1/20
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human STAT1
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 87 kDa; Observed MW: 87 kDa
Gene Name	signal transducer and activator of transcription 1
Database Link	Entrez Gene 6772 Human UniProt ID P42224
Background	Swiss-Prot Acc.P42224.Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
Synonyms	DKFZp686B04100; ISGF-3; OTTHUMP00000205845; STAT91
Reference Data

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