EMA (MUC1) Rabbit Monoclonal Antibody [Clone ID: MC5]

Specifications

Product Data
Clone Name	MC5
Applications	ELISA, IHC, IP, WB
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	Human breast epithelial mucin.
Specificity	This antibody is specific for the amino acid sequence DTRPAP of MUC1. The alpha subunit of MUC1 has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit of MUC-1 contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity. In an attempt to develop a reliable way to detect breast cancer, immunoprecipitation was preformed on NPGP from HMFG using the mouse version of this antibody bound to Sepharose 4B. Furthermore, this antibody was tested in an radioimunnology experiment on NPGP using the mouse version of this antibody (Ceriani et al, 1992; pmid:1377884). The epitope of this antibody was mapped by ELISA using small peptides - the murine version of this antibody was used (Petrakou et al, 1998 ; pmid:9422085). This antibody was used to identify cells expressing the MUC1 protein. This was accomplished by using the mouse version of this antibody on cell lysates of cell transfected with the MUC1 protein in a western blot analysis (Rubinstein et al, 2006; pmid:17145869). To compare the diagnostic usefulness of different antibodies, the mouse version of this antibody was used for immunohistochemistry on cell pellets from pleural fluid specimens (Creaney et al, 2008; pmid:18454162). While developing an antibody against the alpha/beta junction of MUC1 the mouse version of this antibody was used for western blot on the MUC1 protein (Rubinstein et al, 2008; pmid:18821582). While assesing a panel of 56 anti MUC1 antibodies for their ability to detect cancer the mouse version of this antibody was used for an immunoradiometric assay on a serum made from sera from 10 different patients with breast, ovarian or colon cancer (Norum et al, 1998; pmid:9422099). To more accurately determine the epitope of this antibody, an ELISA was preformed using the murine version of this antibody on different small pepetides from the MUC1 protein (Karsten et al, 2004; pmid:15115750).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	mucin 1, cell surface associated
Database Link	Entrez Gene 4582 Human UniProt ID P15941
Synonyms	CD227; EMA; episialin; H23AG; KL-6; MAM6; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; PEM; PEMT; PUM
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.