p21 Ras (HRAS) Rat Monoclonal Antibody [Clone ID: Y13-238]

Specifications

Product Data
Clone Name	Y13-238
Applications	IP
Reactivity	Human, Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.
Specificity	The antibody reacts with v-H-ras (within amino acids 120-138) and rodent c-H-ras p21′s but not v-K-ras or rodent c-K-ras p21′s. This antibody also recognizes human c-H-ras and c-K-ras p21′s, but not human c-N-ras. Y13-238 has been used to immunoprecipitate p21 from human peripheral blood lymphoblasts in order to measure its activation state (Downward 1990). Y13-238 has been used as an anti-ras non-neutralising scFv (Cochet 1998).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	Harvey rat sarcoma viral oncogene homolog
Database Link	Entrez Gene 3265 Human UniProt ID P78460
Synonyms	C-BAS/HAS; C-H-RAS; C-HA-RAS1; CTLO; H-Ras-1; H-RASIDX; Ha-Ras; HAMSV; HRAS1; K-RAS; N-RAS; OTTHUMP00000162769; OTTHUMP00000166053; OTTHUMP00000166055; p21ras; RASH1
Reference Data

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