IL10 Rat Monoclonal Antibody [Clone ID: 9D7]
CNY 7,170.00
货期*
6周
规格
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经常一起买 (1)
beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
CNY 300.00
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Specifications
Product Data | |
Clone Name | 9D7 |
Applications | ELISA, IF, IHC, IP, Neutralize |
Reactivity | Human |
Host | Rat |
Clonality | Monoclonal |
Immunogen | Rats are immunised with synthetic oligopeptide (containing an N-terminal cysteine)-carrier protein conjugates. IL-10 was conjugated to JES3. The mouse non-secreting myeloma fusion partner P3X63-AG8.653, is fused to immunised rat splenocytes to produce heterohybridomas secreting rat antibody. Bioassays were used to identify neutralising mAbs, and immunochemical screens were used to identify mAbs that bind the cytokine. |
Specificity | The six 9D7 Fab CDR loops interact with a discontinuous IL-10M1 epitope comprised of residues 71-83 and 125-137. 9D7 non-competitively antagonises IL-10R1 binding. 9D7 has been used in ELISA experiments in order to specifically detect IL-10 and show higher circulating levels of IL-10 in patients with acute EBV-induced mononucleosis (Taga 1995). Further, 9D7 has been used to immunoprecipitate IL-10 from the supernatants of melanoma cells in order to study the production of IL-10 by melanoma cells (Qiyuan Chen 1994). A fab fragment derived from 9D7 has been used to study the noncompetitive antibody neutralisation of IL-10 by x-ray crystallography (Josephson 2002). |
Formulation | PBS with 0.02% Proclin 300. |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage Condition | Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles. |
Gene Name | interleukin 10 |
Database Link | |
Synonyms | CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF |
Reference Data |
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