CD14 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: To detect Human sCD14 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human sCD14 as a capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Human sCD14. Western Blot To detect Human sCD14 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sCD14 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced from sera of rabbits immunized with highly pure Recombinant Human sCD14. Anti-Human CD14-specific antibody was purified by affinity chromatography and then biotinylated.
Concentration	lot specific
Purification	CD14-specific antibody was purified by affinity chromatography and then biotinylated
Conjugation	Biotin
Storage Condition	Store at -20°C as received.
Database Link	UniProt ID P08571
Background	CD14 is a cell surface-anchored glycoprotein that is expressed predominantly by monocytes and tissue macrophages. CD14 associates with MD-2 (LY-96) and TLR4 to form a receptor complex, which signals specifically in response to bacterial lipopolysaccharide (LPS) binding. The CD14/MD-2/TLR4 receptor complex signals via MyD88, TIRAP, and TRAF6, and ultimately activates NF-κB. CD14 also exists in a soluble form, designated as sCD14, which is capable of specifically binding LPS in the extracellular space. Recombinant Human sCD14 is a 331 amino acid glycoprotein comprising the extracellular portion of the CD14 receptor. The calculated molecular weight of Recombinant Human sCD14 is 35.6 kDa.
Reference Data

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