PTPN6 Mouse Antibody [Clone ID: M160]

Specifications

Product Data
Clone Name	M160
Applications	ICC, IP, WB
Recommend Dilution	WB: 1:500 ICC: 1:100
Reactivity	Human, Rat, Mouse
Host	Mouse
Immunogen	Clone (M160) was generated from a recombinant protein containing amino acids in the C-terminal region of human SHP1. This sequence is highly conserved in rat and mouse SHP1.
Specificity	The antibody detects a 68 kDa* protein in human A431 and Jurkat cells, and does not cross-react with SHP2.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein A Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	68
Database Link	UniProt ID P29350
Background	SHP1 (PTP1C, SH-PTP1, or HCP) is a protein-tyrosine phosphatase (PTP) involved in cell migration, cell proliferation, and immune cell function. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP1 associates with a variety of cytokine and growth factor receptors and regulates signal transduction through dephosphorylation of these receptors or their downstream effectors. Downstream of receptor activation, SHP1 regulates the transcriptional activity stimulated by JAK/Stat and MAPK pathways. SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2. Serine phosphorylation at Ser-591 is mediated by PKCα and leads to inhibition of phosphatase activity. Thus, phosphorylation at tyrosine relative to serine residues may be regulated by different cell signaling pathways to control SHP1 activity.
Note	Protein G purified tissue culture supernatant.
Reference Data

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