MEK1 (MAP2K1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:1000~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Serine 298.
Specificity	MEK1 (Phospho-S298) polyclonal antibody detects endogenous levels of MEK1 protein only when phosphorylated at Ser298.
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 45 kDa
Gene Name	mitogen-activated protein kinase kinase 1
Database Link	Entrez Gene 5604 Human UniProt ID Q02750
Background	MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.
Synonyms	Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP2K1; MAPK/ERK kinase 1; MAP kinase kinase 1; MAPKK 1; MEK-1; MEK 1; MKK1; PRKMK1
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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