BCKDH kinase (BCKDK) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:500~1:1000 IHC: 1:50~1:200 IP 1:50 - 1:100
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 21-70 of Human BCKDK.
Specificity	BCKDK (E41) polyclonal antibody detects endogenous levels of BCKDK protein.
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 46 kDa
Gene Name	branched chain ketoacid dehydrogenase kinase
Database Link	Entrez Gene 10295 Human UniProt ID O14874
Background	BCKDK (branched chain ketoacid dehydrogenase kinase), also known as BCKDHKIN, is a 412 amino acid mitochondrial matrix protein that exists as a monomer and contains one histidine kinase domain. Expressed ubiquitously, BCKDK catalyzes the ATP-dependent phosphorylation and subsequent inactivation of the branched-chain α-ketoacid dehydrogenase (BCKD) complex, a regulatory enzyme complex that plays a crucial role in the catabolic pathways of valine, leucine and isoleucine. Specifically, the BCKD complex functions as the second enzyme in branched-chain amino acid (BCAA) catabolism, effectively catalyzing the irreversible oxidative decarboxylation of BCAAs. Due to the ability of BCKDK to regulate the activity of the BCKD complex, BCKDK plays an essential role in the catabolic pathways of branched-chain amino acid metabolism.
Synonyms	BCKD-kinase; BCKDHKIN; Bckdk; BCKD kinase; Branched-chain alpha-ketoacid dehydrogenase kinase; Branched chain alpha keto acid dehydrogenase kinase; Branched chain ketoacid dehydrogenase kinase; mitochondrial
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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