pgmB Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:1,000 - 1:5,000 ELISA: 1:10,000 - 1:40,000
Reactivity	Lactococcus lacti
Host	Goat
Clonality	Polyclonal
Immunogen	Beta Phosphoglucomutase [Lactococcus lacti]
Specificity	Beta-Phosphoglucomutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified b -Phosphoglucomutase [Lactococcus lacti]. Cross reactivity against b -Phosphoglucomutase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P71447
Background	Beta-Phosphoglucomutase catalyzes the interconversion of D-glucose 1-phosphate and D-glucose 6-phosphate, forming beta-D-glucose 1,6-(bis)phosphate as an intermediate. The beta-phosphoglucomutase acts on the beta-C1 anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose. This enzyme belongs to the family of isomerases, specifically the phosphotransferases (phosphomutases), which transfer phosphate groups within a molecule. The systematic name of this enzyme class is beta-D-glucose 1,6-phosphomutase. This enzyme participates in starch and sucrose metabolism.
Synonyms	goat anti-Beta-Phosphoglucomutase Antibody HRP Conjugation, peroxidase conjugated goat anti-Beta PGM antibody, Glucose phosphomutase 1 antibody, Glucose phosphomutase antibody, PGM antibody, pgmB antibody
Note	Anti-Beta-Phosphoglucomutase has been assayed against 1.0 ug of b-Phosphoglucomutase [Lactococcus lacti] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 is suggested for this product.
Reference Data

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