CHOX_ALCSP Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:1,000 ELISA: 1:1,000 - 1:2,500
Host	Goat
Clonality	Polyclonal
Immunogen	Choline Oxidase [Alcaligenes species]
Specificity	Anti-Choline Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species].  Cross reactivity against Choline Oxidase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P16101
Background	Choline Oxidase is a flavoprotein responsible for the catalyzing the conversion of choline to betaine aldehyde in animals and bacteria. Anti-Choline Oxidase antibody is ideal for researchers interested in Metabolism and Signal Transduction research.
Synonyms	goat anti-Choline Oxidase Antibody biotin Conjugation, biotin Conjugated goat anti-Choline Oxidase Antibody, codA antibody
Note	Anti-Choline Oxidase has been tested by ELISA and western blot. This product is suitable to be assayed against 1.0 ug of Choline Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reference Data

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