P2OX Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:5,000 ELISA: 1:5,000 - 1:20,000
Reactivity	Microbial
Host	Goat
Clonality	Polyclonal
Immunogen	Pyranose Oxidase [E.coli]
Specificity	Anti-Pyranose oxidase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyranose Oxidase [E.coli].  Cross reactivity against Pyranose Oxidase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P79076
Background	Anti-Pyranose oxidase recognizes the oxidoreductase pyranose oxidase. In general, pyranose catalyzes the oxidation of aldopyranoses at the carbon 2 position to form 2-ketoaldoses . Notably, pyranose oxidase catalyzes the conversion of D-glucose and oxygen to 2-dehydro-D-glucose and hydrogen peroxide using flavin adenine dinucleotide (FAD) as a cofactor. Pyranose oxidase also plays a role in the pentose phosphate pathway.
Synonyms	goat anti-Pyranose Oxidase Antibody, biotin Conjugated goat anti-Pyranose Oxidase Antibody, P2Ox, Pyranose oxidase, PROD, POD, POx, Pyranose:oxygen 2-oxidoreductase, Glucose 2-oxidase, FAD-oxidoreductase
Note	Anti-Pyranose oxidase antibody has been assayed against 1.0 µg of Pyranose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:70,000 to 1:350,000 of the reconstitution concentration is suggested for this product.
Reference Data

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