zwf Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:500 - 1:3,000 ELISA: 1:5,000 - 1:25,000
Reactivity	Leuconostoc mesenteroides
Host	Goat
Clonality	Polyclonal
Immunogen	Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides]
Specificity	Anti-GLUCOSE-6-PHOSPHATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides].  Cross reactivity against Glucose-6-Phosphate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P11411
Background	Glucose-6-Phosphate Dehydrogenase catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. It can utilize either NADP+ or NAD+. This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Synonyms	goat anti-Glucose-6-Phosphate Dehydrogenase Antibody, G6PD antibody, G6PD1 antibody, G6pdx antibody, Glucose 6 phosphate 1 dehydrogenase antibody, MET19 antibody, POS10 antibody, Zwf1p antibody
Note	Anti-Glucose-6-Phosphate Dehydrogenase Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Researchers should determine optimal titers for other applications.
Reference Data

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