lacZ Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	WB: User Optimized IF: User Optimized
Host	Rabbit
Clonality	Polyclonal
Immunogen	Beta Galactosidase (E. coli)
Specificity	Anti-Beta Galactosidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and purified and partially purified Beta Galactosidase (E. coli).
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	FITC
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P00722
Background	Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.
Synonyms	rabbit anti-Beta Galactosidase Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Beta Galactosidase Antibody, rabbit anti-beta gal FITC conjugated antibody, β-Gal Fluorescein, Anti-β-Gal Fluorescein Antibody
Note	Anti-Beta Galactosidase Fluorescein Conjugated Antibody has been tested by dot bot and western blot and is designed for fluorescent western blotting and immunofluorescence. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.