Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF
Recommend Dilution	IF: 1:50-1:250 FC: 1:200-1:1,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Protein G [Streptococcus species]
Specificity	Anti-Protein G Texas Red Conjugated Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein G [Streptococcus species], anti-Rabbit IgG and anti-Rabbit Serum.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0mg/mL - lot specific
Conjugation	Texas Red
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Background	Protein G is a protein that has the property of binging to immunoglobulins. It is a 65-kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins.
Synonyms	rabbit anti-Protein G Antibody Texas Red™ Conjugation, Texas Red™ conjugated rabbit anti-Protein G Antibody, Protein G Texas Red™
Note	This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reference Data

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