AAO Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:2,000 - 1:10,000 ELISA: 1:25,000 - 1:125,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Ascorbate Oxidase [cucurbita]
Specificity	Anti-Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita]. Cross reactivity against Ascorbate Oxidase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Database Link	UniProt ID P24792
Background	Ascorbate Oxidase may be involved in a redox system involving ascorbic acid. Ascorbate Oxidase catalytic activity is 4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O.
Synonyms	rabbit anti-Ascorbate Oxidase Antibody, L-ascorbate oxidase, Ascorbase, ASO
Note	Anti-Ascorbate Oxidase has been tested by ELISA and western blot and is suitable for use in immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.
Reference Data

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