ansB Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:500 ELISA: 1:1,000
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	Asparaginase [E. coli]
Specificity	Anti-L-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E. coli]. Cross reactivity against Asparaginase from other sources may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P00805
Background	Anti-L-Asparaginase antibody recognizes the protein asparaginase. Asparaginase is responsible for the catalysis of asparagine to L-aspartic acid and ammonia. In food processing, asparaginase is used to prevent the formation of acrylamide, a carcinogen, from the heating of asparagine which causes it to undergo the maillard reaction. Asparaginase is also used in cancer therapy since leukemic cells are unable to produce their own asparagine and therefore, must rely on circulating asparagine. Treatment with asparaginase converts the asparagine to aspartic acid denying the leukemic cells of asparagine, resulting in their death. Anti-L-Asparaginase antibody is suitable to investigators interested in cancer, metabolism, and enzymology.
Synonyms	rabbit anti-L-Asparaginase Antibody biotin Conjugation, biotin Conjugated rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody
Note	Anti-L-Asparaginase Biotin Conjugated Antibody has been by ELISA, dot blot, and western blot. This product is assayed against 1.0 ug of Asparaginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,200 to 1:12,800 of the reconstitution concentration is suggested for this product.
Reference Data

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