faoxC Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	WB: 1:500 - 1:5,000 IHC: 1:100 ELISA: 1:5,000 - 1:20,000
Reactivity	Corynebacterium sp
Host	Sheep
Clonality	Polyclonal
Immunogen	Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]
Specificity	Anti-FRUCTOSYL-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase [E.coli].  Cross reactivity against Fructosyl-Amino Acid Oxidase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.1 mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID Q8RIU8
Background	Fructosyl amino acid oxidase is a flavoprotein that catalyzes the oxidation of fructosyl amino acids to form glucosone, amino acid and hydrogen peroxide. Anti-Fructosyl-amino acid oxidase can be useful for researchers interested in diabetes research.
Synonyms	sheep anti-Fructosyl-Amino Acid Oxidase Antibody, Fructosyl-amino acid oxidase
Note	Fructosyl-amino acid oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Fructosyl-Amino Acid Oxidase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Reference Data

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