DsRed Rabbit Polyclonal Antibody

CAT#: TA397545S

Anti-mCherry Antibody Pre-Adsorbed

Size: 100 ug 25 ul



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CNY 2,119.00


货期*
4周

规格
    • 25 ul

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Specifications

Product Data
Applications ELISA, IF, IHC, WB
Recommend Dilution WB: 1:2,000 - 1:10,000
IHC: 1:200 - 1:2,000
IF: 1:200 - 1:2,000
ELISA: 1:150,000 - 1:250,000
Reactivity mCherry, RFP, rRFP
Host Rabbit
Clonality Polyclonal
Immunogen The immunogen is a mCherry mutant variant fusion protein of RFP corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Specificity mCherry was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Expect reactivity against mCherry, RFP and its variants: tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified mCherry.  No reaction was observed against Human, Mouse or Rat serum proteins.  ELISA was used to confirm specificity at less than 0.1% of target signal.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 1.04 mg/mL - lot specific
Conjugation Unconjugated
Storage Condition Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background mCherry antibody is ideal for western blotting. Fluorescent proteins such as Discosoma Red Fluorescent Protein (and its variants), and GFP are widely used in research practice. Both commonly serve as a markers for gene expression and protein localization. DsRed was isolated from sea anemone Discosoma sp. mushroom and GFP is originated from Aequorea victoria jellyfish. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional steps in the chromophore maturation and obligates tetrameric structure. All mCherry antibodies have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein.
Synonyms rabbit anti-mCherry antibody, RFP, mCherry monomeric red fluorescent protein, Red Fluorescent Protein (RFP), rDsRed, Discosoma sp. Red Fluorescent Protein
Note Polyclonal anti-mCherry is designed to detect mCherry, RFP, and its variants. Anti-mCherry (Discosoma sp.) has been tested by ELISA and Western blot and is intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS). Researchers should determine optimal titers for applications that are not stated. In addition, we performed conjugation of RFP antibodies to either fluorescent dyes, biotin or horseradish peroxidase to further facilitate RFP protein detection and quantification.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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