ERCC1 Mouse Monoclonal Antibody [Clone ID: 2E12]

Specifications

Product Data
Clone Name	2E12
Applications	10k-ChIP, IF, IHC, WB
Recommend Dilution	WB 1:500~1000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human ERCC1 (NP_973730) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	32.4 kDa
Gene Name	ERCC excision repair 1, endonuclease non-catalytic subunit
Database Link	NP_001974 Entrez Gene 13870 MouseEntrez Gene 292673 RatEntrez Gene 574267 MonkeyEntrez Gene 2067 Human UniProt ID P07992
Background	The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq]
Synonyms	COFS4; RAD10; UV20
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Nucleotide excision repair

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