Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
CAT#: AP21485AF-N
Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free
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CNY 7,161.00
货期*
5周
规格
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Specifications
Product Data | |
Product Name | Mouse IgE (Fc specific) Rabbit Polyclonal Antibody |
Applications | ELISA, ID, IF, IP, WB |
Recommend Dilution | Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
Reactivity | Mouse |
Host | Rabbit |
Immunogen | Purified homogenous IgE isolated from Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Formulation | PBS, pH 7.2 without preservatives or foreign proteins. State: Azide Free State: Lyophilized hyperimmune purified IgG fraction |
Concentration | 10.0 mg/ml |
Purification | Hyperimmune antisera with strong precipitating activity are selected for Fractionation and purification by of the IgG fractionn by DEAE Chromatography |
Conjugation | Unconjugated |
Storage Condition | Prior to reconstitution store at 2-8°C. |
Note | Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity reacting with other components of the immunoglobulin system and to any other serum protein. Special attention is given to the elimination of antibodies to the common Fab portion of immunoglobulins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
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