Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
CAT#: AP31795AP-N
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP
Conjugation: Unconjugated Agarose Biotin FITC HRP
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CNY 3,190.00
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Specifications
Product Data | |
Product Name | Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody |
Applications | ELISA, WB |
Recommend Dilution | ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate. Western Blot (1/1000). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA. |
Reactivity | Chicken |
Host | Goat |
Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
Formulation | 50mM HEPES, pH 7.0, 0.1M NaCl, 1.0mM MgCl2, 0.1mM ZnCl2 containing 0.2% BSA as stabilizer and 0.02% Sodium Azide as preservative Label: AP State: Liquid purified IgY fraction Label: Alkaline Phosphatase |
Concentration | lot specific |
Purification | Affinity Chromatography |
Conjugation | AP |
Storage Condition | Store the antibody undiluted at 2-8°C in the dark. |
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