Human IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody
CAT#: R1334B
Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin
Conjugation: Unconjugated FITC HRP
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CNY 3,938.00
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Specifications
Product Data | |
Product Name | Human IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody |
Recommend Dilution | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:17,000 to 1:75,000 of the reconstitution concentration is suggested for this product. |
Reactivity | Human |
Host | Rabbit |
Immunogen | Human IgG, whole molecule. |
Formulation | 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Thimerisol as preservative. Label: Biotin State: Lyophilized purified Ig fraction. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule. |
Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity chromatography. |
Conjugation | Biotin |
Storage Condition | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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