Sheep IgG F(c) specific Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Sheep IgG F(c) specific Rabbit Polyclonal Antibody
Recommend Dilution	Suitable for Western blotting (1:1,000-1:10,000), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry (1:500-1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Reactivity	Sheep
Host	Rabbit
Immunogen	Sheep IgG F(c) fragment.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative. Label: HRP State: Lyophilized purified Ig fraction. Label: Horseradish Peroxidase
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	HRP
Storage Condition	Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 4°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.