Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed Rabbit Polyclonal Antibody
CAT#: R1403B
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Biotin
Conjugation: Unconjugated FITC HRP
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CNY 3,694.00
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Specifications
Product Data | |
Product Name | Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed Rabbit Polyclonal Antibody |
Recommend Dilution | Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:70,000 of the reconstitution concentration is suggested for this product. |
Reactivity | Mouse |
Host | Rabbit |
Immunogen | Mouse IgG whole molecule |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer Label: Biotin State: Lyophilized F(ab')2 fragments. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Rabbit IgG F(ab')2 molecule |
Concentration | lot specific |
Purification | Immunoaffinity chromatography. |
Conjugation | Biotin |
Storage Condition | Store vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. |
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