Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed Rabbit Polyclonal Antibody
Recommend Dilution	Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reactivity	Mouse
Host	Rabbit
Immunogen	Mouse IgG whole molecule
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; 0.01% (w/v) Gentamicin Sulfate Label: HRP State: Lyophilized Ig fraction Label: Horseradish Peroxidase (Molecular Weight 40,000 daltons)
Concentration	lot specific
Purification	Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Conjugation	HRP
Storage Condition	Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.