Rat IgG (H+L chain), F(ab)2 Fragment Rabbit Polyclonal Antibody
CAT#: R1413B
Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Biotin
Conjugation: Unconjugated FITC HRP
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CNY 4,988.00
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Specifications
Product Data | |
Product Name | Rat IgG (H+L chain), F(ab)2 Fragment Rabbit Polyclonal Antibody |
Recommend Dilution | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilution(s): This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested. |
Reactivity | Rat |
Host | Rabbit |
Immunogen | Rat IgG whole molecule |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide preservative . Label: Biotin State: Lyophilized F(ab')2 fragments. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Rabbit IgG molecule. |
Concentration | lot specific |
Purification | Immunoaffinity chromatography. |
Conjugation | Biotin |
Storage Condition | Store vial at 2-8°C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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